Friday, August 12, 2011

My DNA shoots out of the wells in my Agarose Gel... why?

I have DNA that I'm cutting with BamH1 and Pml1. They use different buffers so I do a PCR purification in between each digestion. After an hour each of digest, I add the correct amount of loading buffer to my samples and load my 1.3% agarose gel in 1X TAE buffer. After loading the gel, my samples quickly disperse out of the well. The wells are definately deep enough. It's like the sample is "running away" from the agarose. Earlier today I used the SAME running buffer, and SAME agarose and the SAME box to run some samples that worked just fine. This happened to me before with BamH1 and another restriction enzyme. Do you know what's going on???

No comments:

Post a Comment